Download Advances in Chromatography: Volume 41 by Phyllis R. Brown, Eli Grushka PDF

By Phyllis R. Brown, Eli Grushka

An up to date reference, delivering an leading edge viewpoint on breakthroughs in separation technological know-how, corresponding to reverse-phase HPLC, advances in hyphenation, and linear dependence of relative retention values at the nature of the provider fuel and commonplace column strain in gas-liquid chromatography.

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95] have chosen another approach to perform CEC with mobile phases of low pH. They have used a mixed-mode phase that contains octadecyl and dialkylamino groups (reversed-phase/ anion-exchange phase). The amino groups determine at low pH the charge density at the surface and the direction of the electroosmotic flow is reversed with respect to the direction found with bare silica gel. Huang et al. have demonstrated that this phase is ideally suited for the separation of (positively charged) peptides.

4] is not dependent on the particle diameter. Knox [44] has predicted that, using submicrometer material, all contributions from the A and C terms of the plate-height equation become insignificant compared to the band-broadening process, due to axial diffusion. 8 µm with very narrow size distribution is one approach to obtain extremely high efficiency in CEC, the use of widepore material and pore flow effects is another interesting approach. Li and Remcho [45] report results obtained with octadecyl silica gels with mean pore diameters ranging from 6 to 400 nm.

10 / Pyell Fig. 5 Flow profiles generated by (a) hydrodynamic flow, (b) electroosmosis in two capillaries (1) and (2) of different inner diameter (velocities of liquid segments are represented by arrows). III. THEORY A. ’’ No studies are available concerning the accuracy of those measurements. ’’ Generally, the so-determined linear velocity is treated as an equivalent of the electroosmotic velocity veo. However, in the case of on-column detection (open section of the capillary behind the chromatographic bed, see Fig.

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