By P. D. Evans
Insect body structure is at the moment present process a revolution with the elevated program of molecular organic options to enquire the molecular mechanisms underlying the physiological responses to insect cells. Advances in Insect body structure has instituted a dedication to the e-book of top of the range stories on molecular biology and molecular genetics in components the place they supply an elevated realizing of physiological strategies in bugs. quantity 25 includes elevated assurance at the molecular biology of insect body structure.
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Extra info for Advances in Insect Physiology, Vol. 25
One late polypeptide was shown to migrate with the IV22 major capsid protein. Very little has been published on the molecular aspects of insect IV replication. Instead, comparisons are always drawn with the more extensively studied replication cycle of the vertebrate IV, frog virus 3 (FV3); however, the relevance of the FV3 replication cycle to the insect IVs is not known (Kelly, 1985; Ward and Kalmakoff, 1991). , 1991). Virus uptake into cells is by pinocytosis and incorporation into phagocytic vesicles, in which the virus particle is uncoated.
Foreign genes are inserted directly under the p10 promoter by co-transfection of insect cells with a p10 transfer vector and purified wild-type AcMNPV DNA. Other AcMNPV gene promoters may also be used for the expression of foreign genes in virus-infected cells. Several late gene promoters, while not as active as the very late polyhedrin and p10 gene promoters, can produce useful quantities of recombinant material. One problem encountered when using the late gene promoters is that they are normally associated with genes responsible for producing virus particle structural proteins.
A few, limited experiments have been undertaken to discern the ability of EPVs to replicate in vertebrate cells. In murine L-929 cells inoculated with AmEPV, no virus-induced proteins were detected at 37°C using [35S]methionine pulse-labelling. However, in E. acre0 (EAA-BTI) cells inoculated with vaccinia virus, an increase in protein production was detected by ELISA using antiserum raised against purified vaccinia virus (Langridge, 1983b). L. A. KING eta/. 6 BIOLOGICALCONTROL Because of their similarities to the vertebrate poxviruses, few serious studies have reported the use of EPVs as biological control agents.