By Weiming Xia, Huaxi Xu
Within the look for an efficient remedy for Alzheimer's disorder, APP is a special version protein that illustrates the good selection of simple and complicated characterization recommendations to be had. Exploring quite a few organic concepts to explain the constitution and serve as of this transmembrane protein, this article offers every one strategy with designated, step by step protocols to accomplish reproducible effects and supply a framework for learning different membrane proteins.
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Extra resources for Amyloid Precursor Protein: A Practical Approach
32. A. C. Amyloid precursor protein modulates the interaction of nerve growth factor with p75 receptor and potentiates its activation of trkA phosphorylation. Mol. Brain. Res. 56, 125, 1998. 33. Henry A. et al. Inhibition of platelet activation by the Alzheimer’s disease amyloid precursor protein. Br. J. Haematol. 103, 402, 1998. 34. J. MOLSCRIPT: a program to produce both detailed and schematic plots of proteins. J. Appl. Crystallogr. 24, 946, 1991. fm Page 41 Wednesday, November 3, 2004 3:20 PM 4 Analysis of Amyloid Precursor Protein Processing Protease β-Secretase: Tools for Memapsin 2 (β-Secretase) Inhibition Studies Gerald Koelsch, Vajira Weerasena, Dongwoo Shin, Arun K.
5 for 3 min each and discard the supernatants. 5. Wash the cells twice with ice cold binding medium, then add prewarmed medium and return cells to the 37˚C incubator. 6. Collect the medium after 5 to 30 min. Precipitate the medium with TCA. 7. 2 N NaOH. 8. Measure the radioactivity of both fractions in a gamma counter. fm Page 25 Wednesday, November 3, 2004 2:59 PM To obtain the specific binding, the radioactivity from untransfected control cells is subtracted from the counts resulting from the transfected cells.
Identification, biogenesis and localization of precursors of Alzheimer’s disease A4 amyloid protein. Cell 57, 115–126, 1989. 35. Oltersdorf, T. et al. The Alzheimer amyloid precursor protein: identification of a stable intermediate in the biosynthetic/degradative pathway. J. Biol. Chem. 265, 4492–4497, 1990. 36. Haass, C. et al. Swedish mutation causes early-onset AD by β-secretase cleavage within the secretory pathway. Nature Med. 1, 1291–1296, 1995. fm Page 19 Wednesday, November 3, 2004 2:59 PM 2 Assays for Analysis of APP Secretion and Recycling Markus P.